Table 3. Minimum inhibitory concentration (MIC; μg ml-1) of β-lactams on recombinant E. coli cells harboring constructs from this work.
Construct a | Antibiotic b | |||||||
---|---|---|---|---|---|---|---|---|
Amo | Amp | Cfm | Cft | Cfx | Cph | Crb | Pip | |
βLR16 in EPI300 | 4 | 4 | <0.5 | 1 | 4 | 4 | 64 | 4 |
pCF430 in EPI300 | 2 | 2 | <0.5 | <0.5 | 2 | 2 | 8 | 2 |
pCRC03UVRA in EPI300 | 4 | 4 | 1 | 1 | 8 | 4 | 128 | 4 |
pUVRA28b in EPI300 | 2 | 4 | <0.5 | <0.5 | 2 | 4 | 8 | 4 |
pCRC03UVRA in BL21(DE3) | 8 | |||||||
pUVRA28b in BL21(DE3) | 2 | |||||||
βLR16 in BW25113 | 32 | |||||||
pCF430 in BW25113 | 8 | |||||||
βLR16 in ΔompF | 32 | |||||||
pCF430 in ΔompF | 16 | |||||||
βLR16 in ΔompC | 16 | |||||||
pCF430 in ΔompC | 2 |
a pCRC01UVRA showed no difference in levels of Crb resistance compared to vector alone (pUVRA28b), while pCRC02UVRA showed the same Crb resistance level as pCRC03UVRA. ΔompF and ΔompC are in the BW25113 E. coli background and are from the Keio collection of gene knockouts (see Table 1).
b Antibiotic abbreviations are as follows: Amo, Amoxicillin; Amp, ampicillin; Cfm, cefamandole; Cft, ceftazidime; Cfx, cefoxitin; Cph, cephalexin; Crb, carbenicillin; Pip, piperacillin.