Figure 1.

Elevated locomotor activity and sleep loss in adult A. mexicanus cavefish . (A) Total swimming distance (meters) is significantly greater in Pachón cavefish (red line) during the day (unshaded; ZT 0 to 14) and night (shaded; ZT 14 to 24) than surface fish (black line). Bars at each 10 minutes represent mean + standard error of mean (s.e.m.). (B) Quantification of total 24 hour activity reveals significantly enhanced locomotor activity in Pachón cavefish (U = 373, P <0.001). Each dot represents the individual phenotypic value. Black horizontal and vertical lines indicate mean ± s.e.m. (C, D) Sleep profile and total 24 hour sleep reveal reduced sleep in Pachón cavefish (red) compared to surface fish (black). (t₈₃ = 4.3, P <0.001). (E, F) Averaged waking activity in each 10 minutes does not differ between surface fish (black) and Pachón cavefish (red) (U = 767, P = 0.232). N = 42, 43 for surface fish and Pachón cavefish, respectively, for all assays. Sf: surface fish. Pa: Pachón cavefish. *** denotes P <0.001. n.s.: not significant.