Fig 3. Stoichiometry of H3/H4-Asf1 for K9 and K23 in the presence of fixed concentrations of saturating acetyl-CoA and H3/H4.
The detailed experimental conditions are described in the section “Stoichiometry”. The error bar represents the standard error in kcat(app). Panels (A) and (B) show that the acetylation rates of H3K9 and H3K23, respectively, increase as a function of molar ratio of Asf1 to H3/H4, before all H3/H4 binds with Asf1. The inflection points of H3K9 and H3K23 are 1.21 and 1.09, respectively, indicating that the binding stoichiometry of Asf1 and H3/H4 dimer is approximately 1:1.