Table 1. Steady-state parameters from kinetic assays of histone titration (mean ± standard error).
Substrate Titrated | Residue Acetylated | K(app)(μM) | kcat(app)(x 10-2 s-1) | kcat/Km(x 10-2 μM-1s-1) |
---|---|---|---|---|
H3 | H3K9 | 1.2 ± 0.3 | 24.0 ± 1.1 | 19.4 ± 5.2 |
H3 | H3K23 | 2.9 ± 0.6 | 14.6 ± 0.7 | 5.0 ± 1.1 |
H3/H4 | H3K9 | 0.9 ± 0.3 | 1.5 ± 0.07 | 1.8 ± 0.5 |
H3/H4 | H3K23 | 0.6 ± 0.2 | 1.2 ± 0.06 | 1.9 ± 0.6 |
H3/H4-Asf1 | H3K9 | 1.9 ± 0.5 | 6.2 ± 0.4 | 3.4 ± 1.0 |
H3/H4-Asf1 | H3K23 | 2.1 ± 0.4 | 5.6 ± 0.3 | 2.6 ± 0.5 |