Table 3. Catalytic proficiency of Rtt109-Vps75-mediated acetylation from histone titration (mean ± standard error).
Histone Titrated | Residue Acetylated | (kcat/Km)/knE(No unit) | ΔΔG (kcal/mol) |
---|---|---|---|
H3 | H3K9 | (2.8 ± 1.5) x106 | -9.1 ± 0.3 |
H3 | H3K23 | (7.3 ± 3.7) x105 | -8.3 ± 0.3 |
H3/H4 | H3K9 | (2.0 ± 0.7) x105 | -7.5 ± 0.2 |
H3/H4 | H3K23 | (2.1 ± 0.7) x105 | -7.5 ± 0.2 |
H3/H4-Asf1 | H3K9 | (3.7 ± 1.2) x105 | -7.9 ± 0.2 |
H3/H4-Asf1 | H3K23 | (2.9 ± 0.8) x105 | -7.7 ± 0.2 |