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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Microsc Res Tech. 2009 Apr;72(4):323–332. doi: 10.1002/jemt.20655

Table 2.

A) While diffusing or binding the particle has the same brightness.
Nominal time A
(mobile)		 Tau (s) χ2 A
(fixed)		 Tau(s) χ2
0.5 1.545 0.106213 2.87 0.50194 0.054112 3.14
0.05 1.47931 0.072177 1.63 0.48385 0.025532 2.46
0.005 1.379 0.042390 6.56 0.27174 0.006111 7.91
B) While diffusing the particle is 10 times dimmer than when binding.
Nominal time A
(mobile)		 Tau (s) χ2 A
(fixed)		 Tau(s) χ2
0.5 3.142 1.20 0.47 1.363 0.244 0.18
0.05 3.217 0.109 0.55 1.404 0.0603 0.50
0.005 2.317 0.027 3.60 1.300 0.0055 0.80
C) The parameter A is proportional to the inverse of the number of binding sites.
N of sites A Tau (s)
25 2.92 0.0052
50 1.41 0.0052
100 0.736 0.0052
200 0.370 0.0052