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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Neuron. 2014 May 8;82(5):1017–1031. doi: 10.1016/j.neuron.2014.04.016

Figure 2. Warm temperatures activate TRPA1 single-point mutants.

Figure 2

(A) Whole-cell recordings of transiently transfected CHO cells in Ca2+-free buffer. Representative currents evoked by ramp-protocol (±100 mV) for pcDNA, wild-type mouse TRPA1 and mutants S250N, M258L and D261G during a temperature-step, followed by application of MO (100 µM). Numbers indicate basal currents (1), peak-responses (2) recovery currents (3) and mustard-oil application (4). (B) Current-voltage relationships at indicated time points. (C) Average of maximal current amplitudes at ±100 mV for different single-point mutants. Numbers of experiments are indicated above bars. Data represent mean ± s.e. Stars indicate statistical significance as compared to pcDNA (*** p< 0.001, ** p<0.01). (D) Representative Arrhenius plots at ±100 mV. Data represent mean ± stdv. Dotted lines are weighted exponential fits to sections before and after channel activation. N = 9 for mutant S250N, n = 16 for wild-type mouse TRPA1 and n = 4 for pcDNA.