Table 3.

Data and sample size of neurons from primary cultures and neuron–glia cocultures used in degeneration and Sholl analyses

	Degeneration analysis (number of total/degenerated neurons)			Sholl analysis (number of analyzed neurons)
	0.5 mM	1.5 mM	3.0 mM	0.5 mM	1.5 mM	3.0 mM
Primary neurons number of preparations = 3
VSOP-R1	249/83 (n=5)	163/63 (n=5)	195/80 (n=5)	n=55	n=67	n=60
VSOP-R2	294/86 (n=5)	210/74 (n=5)	210/70 (n=5)	n=63	n=61	n=79
Ferucarbotran	221/85 (n=5)	151/66 (n=5)	211/88 (n=5)	n=52	n=47	n=50
Ferumoxytol	246/84 (n=5)	233/89 (n=5)	229/90 (n=5)	n=62	n=50	n=48
Controls		579/129 (n= 6)			n=56
Coculture neurons number of preparations = 4
VSOP-R1	22/3 (n=8)	12/7 (n=6)	22/7 (n=7)	n=13	n=12	n=12
VSOP-R2	17/3 (n=5)	30/7 (n=6)	27/5 (n=7)	n=15	n=14	n=21
Ferucarbotran	21/6 (n=8)	19/5 (n=5)	25/6 (n=7)	n=12	n=11	n=16
Ferumoxytol	38/9 (n=8)	30/8 (n=6)	42/8 (n=8)	n=28	n=17	n=29
Controls		39/10 (n=7)			n=23

Notes: Shown are total numbers of counted neurons in comparison to numbers of degenerated neurons for each exposure condition. The ratio of these numbers as percentages is illustrated in Figure 3 for primary and in Figure 4 for neuron–glia cocultures. Numbers of quantified cells (n) for each exposure condition of independently prepared cultures are shown in brackets. For Sholl analysis, total numbers of analyzed neurons are shown.

Abbreviation: VSOP, very small iron oxide particle.