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. 2014 Dec 11;14:935. doi: 10.1186/1471-2407-14-935

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© Girgert et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Impact of 17β-estradiol and estriol on proliferation of TNBC cells. Cells of a GPR30 positive (HCC1806) and a GPR30 negative TNBC cell line (MDA-MB-231) were grown for 7 days in culture medium supplemented with charcoal-treated FCS either in the presence of 10^-12 M to 10^-6 M 17β-estradiol (A and B) or 10^-6 M to 8×10^-5 M estriol (C and D). Cell number was evaluated in microwell plates by a colorimetric assay using Alamar blue. Optical densities (OD) measured in the non-stimulated wells (control) were set 100%. The ODs estimated in the stimulated wells were divided by the average value of the control wells to give the relative cell number in % of control achieved under the indicated conditions. (a) p < 0.05 vs. control; (b) p < 0.01 vs. control. Data are mean values and SE of three independent experiments with four replicates.