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. 2015 Mar 18;10(3):e0119415. doi: 10.1371/journal.pone.0119415

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© 2015 Kawada et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Gastric cancer cells were cultured for 1 day in Hs738 CM prepared by culturing Hs738 cells with the 1% (left) or indicated concentrations of D-FBS (right) (+) or control medium (-). Ser/Thr-phosphorylated proteins were detected by anti-phospho-Ser/Thr antibody (9614). An arrowhead indicates the position of the band of the right panel. (B) The same cell lysates in (A) were assessed with the indicated antibodies. (C) MKN-7 cells were cultured with or without Hs738 cells for 1 day. The intracellular phosphorylated RPS6 proteins were immunostained. Scale bar is 200 μm.