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. 2015 Mar 18;10(3):e0119415. doi: 10.1371/journal.pone.0119415

Fig 5. Gastric cancer cells secrete PGE2 and TNFα to stimulate IL-6 production by gastric stromal cells.

Fig 5

(A) Hs738 cells were cultured for 1 day in CM prepared from the indicated cells. The amount of IL-6 in the cultured supernatant was determined. The values are means ± s.d. (n = 3). (B) Hs738 cells were cultured with PGE2 for 1 day. The amount of IL-6 in the cultured supernatant was determined. The values are means ± s.d. (n = 3). (C) The amount of PGE2 in CM prepared from the indicated cells cultured for 2 days was determined. The values are means ± s.d. (n = 3). (D) Hs738 cells were cultured with TNFα or IL-1β for 1 day. The amount of IL-6 in CM was determined. The values are means ± s.d. (n = 3). (E) The amounts of TNFα and IL-1β in CM prepared from the indicated cells cultured for 2 days were determined after the concentration of the CM. The values are means ± s.d. (n = 3). *Under detectable level.