Fig 1. Selection of CDI^R mutants of B. thailandensis E264.

A) T23 transposon insertion sites were identified by semi-arbitrary PCR as described in Methods. Orange arrows indicate T23 insertions in the same transcriptional orientation of the disrupted gene and blue arrows indicate insertions in the opposite orientation. The corresponding CDI^R mutant strain number is given above each arrow. Automated gene annotations are given below each ordered locus designation. GT-1, GT-2 and GT-9 indicate predicted glycosyltransferase families and DUF designations indicate domains of unknown function. B) The indicated B. thailandensis strains were co-cultured with Bt81 inhibitors (Table 1) that express the CDI_II ^Bp1026b system for 24 h on solid medium, and the competitive index was calculated as described in Materials and Methods. The strain labeled cdiI ^1026b expresses the cognate CdiI_II ^Bp1026b immunity protein. Data represent the mean ± SEM for three independent experiments. Analysis of the data using Student’s t-test is shown at the top, with bars between samples that were statistically significant (* = p< 0.05).