Table 2. Cell viability of microglial cells after 48 hour incubation with human complement components (C1q, C1 inhibitor, C4, C5 and C9), both individually and in combination with each other; and a human complement standard containing complement components C1q, C2, C3, C4, C5, C6, C7, C8, C9 and factor B.

	Cell Proliferation (abs at 570nm)	Complement C1q	Complement C1 inhibitor	Complement C4	Complement C5	Complement C9	C1q + C4	C5 + C9	C1q, C1inhib, C4, C5 + C9	Complement Standard	Complement Standard NAD concentration (ng)
Control (0μg/μl)	Average	0.317	0.609	0.454	0.375	0.324	0.384	0.356	0.378	0.511	0.114
	SEM	0.019	0.07	0.025	0.031	0.014	0.025	0.018	0.013	0.004	0.003
0.005μg/μl	Average	0.3	0.593	0.458	0.382	0.333	0.377	0.371	0.377	0.426	0.104
	SEM	0.042	0.02	0.019	0.028	0.0098	0.019	0.025	0.019	0.031	0.010
0.025μg/μl	Average	0.31	0.571	0.461	0.364	0.336	0.366	0.359	0.331*	0.210**	0.094
	SEM	0.018	0.03	0.02	0.025	0.0096	0.025	0.021	0.015	0.036	0.063
0.05μg/μl	Average	0.267	0.49	0.435	0.355	0.344	0.289*	0.265*	0.292*	0.129**	0.063*
	SEM	0.0075	0.05	0.019	0.03	0.021	0.027	0.013	0.024	0.018	0.0024

Cell viability was determined by MTT assay of cell proliferation and intracellular NAD levels (for complement standard samples). Replicates included n = 6 for cell proliferation measurements and, n = 3 for NAD concentrations.

* p ≤ 0.05 vs Control,

** p ≤ 0.01 vs Control