Skip to main content
. 2015 Mar 18;10(3):e0120293. doi: 10.1371/journal.pone.0120293

Fig 3. Perithecium formation and gene expression associated with sexual development.

Fig 3

(A) Strains grown on carrot agar media were self-fertilized. Photographs were taken 7 days after sexual induction. Z3643, WT strain; FgWc-1, FgWc-1 deletion mutant; FgWc-2, FgWc-2 deletion mutant; FgFph, FgFph deletion mutant; FgWc-1/2, FgWc-1 and FgWc-2 double deletion mutant; Wc-1c, complemented strain of FgWc-1; Wc-2c, complemented strain of FgWc-2; Fphc, complemented strain of FgFph. The size bar indicates 500 μm. (B) Relative transcript levels for MAT1-1-1, MAT1-2-1 and PKS3. Transcript levels of the genes in each strain were analyzed using qPCR. Total RNAs were extracted from the 5-day-old cultures on carrot agar after perithecial induction. (C) Perithecium formation of F. graminearum strains on complete agar medium. Photographs were taken 7 days after sexual induction. Dissecting the perithecia showed the asci and ascospores of each strain (inset boxes). (D) Relative transcript levels of MAT1-1-1 and MAT1-2-1 from 1- and 4-day-old cultures on complete agar medium after perithecial induction. DAI, day after induction of sexual development.