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. 2015 Mar 18;10(3):e0119625. doi: 10.1371/journal.pone.0119625

Fig 4. Expression of p21.5 interferes with the process of nucleocapsid formation.

Fig 4

HepG2 cells were transduced with Ad1.3HBV and/or AdSS for three days and cell lysates were harvested for sucrose gradient centrifugation (10–60%) and then fractionation was performed. (A) Equal volumes of total protein were separated by electrophoresis on an agarose gel and the expression of nucleocapsid in each fraction was detected by particle blot analysis using an HBx-specific probe. (B) Equal volumes of total protein were separated by electrophoresis on SDS-PAGE and the expression of HBV core protein in each fraction was detected by western blot analysis using an anti-core antibody. (C) The relative proportions of core protein in the fractionated samples (Ad1.3HBV alone or Ad1.3HBV plus AdSS) were estimated and aligned according to the fraction.