Fig 7. HPLC analysis of radioactive steroids formed after a 1-h incubation of frog hypothalamic homogenates with tritiated pregnenolone ([3H]Δ5P) in the absence (A) or presence of 10-6 M etifoxine (B).
The ordinate indicates the radioactivity measured in the HPLC eluent. The dashed lines represent the gradient of secondary solvent (% solution B). The arrows indicate the elution positions of standard steroids: 17OH-Δ 5P, 17-hydroxypregnenolone; DHEA, dehydroepiandrosterone; Δ 4, androstenedione; 17OH-P, 17-hydroxyprogesterone; tetrahydrodeoxycorticosterone, THDOC; P, progesterone; Δ 5P, pregnenolone; DHP, dihydroprogesterone; THP, tetrahydroprogesterone.