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. 2015 Mar 18;10(3):e0119022. doi: 10.1371/journal.pone.0119022

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© 2015 Gai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — PCR analysis of rolB (670 bp), rolC (534 bp), aux1 (350 bp) and virD (438 bp) genes in hairy roots (genomic DNA extracted from the transformed ITHRL V), positive control (Ri-plasmid DNA extracted from A. rhizogenes LBA 9402) and negative control (genomic DNA extracted from I. tinctoria aseptic plantlets). PCR products were analyzed by electrophoresis on a 2.5% (w/v) agarose-ethidium bromide gel along with 1000 bp DNA marker.