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. 2015 Jan 23;14(2):200–208. doi: 10.1111/acel.12294

Figure 3.

Figure 3

Reduction of miR-125b levels in monocytes resulted in increased CCL4 protein expression. (A) A representative graph of CCL4 staining in monocytes transfected with hsa-miR-125b miRCURY™ LNA inhibitor or a scrambled oligonucleotide. (B) Level of CCL4 protein (MFI) in monocytes transfected with hsa-miR-125b miRCURY™ LNA inhibitor or a scrambled oligonucleotide or no oligo. Freshly isolated monocytes were transfected with either hsa-miR-125b miRCURY™ LNA inhibitor (N = 13), or a scrambled oligonucleotide (N = 13), or no oligo (N = 6) for 16 h and followed by LPS stimulation for 3 h. Cells were harvested, and the levels of intracellular CCL4 protein were measured by the mean fluorescence intensity under the gate of positively transfected cells based on the oligonucleotide-linked fluorescein or no gate for no oligo transfection control.