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. 2015 Jan 23;14(2):200–208. doi: 10.1111/acel.12294

Figure 7.

Figure 7

Inhibition of CCL4 expression by miR-125b through a binding site at the 3′UTR of CCL4. (A) The diagram of the putative miR-125b binding site at the 3′UTR of CCL4 and miR-125b binding site deleted mutant. The complementary base pairing between CCL4 3′UTR and miR-125b is predicted by ‘MicroRNA.org’. (B) Inhibition of CCL4 by miR-125b requiring the specific sequence at the 3′UTR of CCL4. miR-125b overexpressed Jurkat cells were further transfected with a renilla luciferase reporter containing either wild-type 3′UTR or miR-125b binding site deleted 3′UTR of CCL4. The renilla luciferase activity was normalized to the constitutively expressed firefly luciferases. The data are presented as the means of three independent experiments and SEM (N = 9, **P < 0.01).