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. 2015 Jan 23;14(2):249–264. doi: 10.1111/acel.12310

Figure 6.

Figure 6

Loss of proteostasis in CMA-deficient mice with age. (A) Immunoblot (IB) of livers from 12- and 24-month-old control (Ctr) and Albumin-Cre:L2Af/f (L2AKO) mice. Right: densitometric quantification, n = 3. (B) LC3 flux in livers from 24 h Ctr and L2AKO mice treated or not with leupeptin 2 h prior to tissue dissection. Bottom: densitometric quantification, n = 3. (C) Autofluorescence in liver sections from 12-month-old Ctr and L2AKO mouse livers. Nuclei are highlighted with DAPI. Insets: higher magnification of boxed regions. Scale bar 10 μm main. (D) IB for ubiquitinated proteins of livers from 24 h starved Ctr and L2AKO mice incubated in the presence or absence of lactacystin. Insoluble proteins are shown in the gel stacking. Bottom: densitometric quantification, n = 3. (E) Filter retardation assay and IB for K48-ubiquitinated proteins from liver homogenates from Ctr and L2AKO mice at the indicated ages. Bottom: quantification of aggregates, n = 6. A.D.U. stands for arbitrary densitometric units. All values are expressed as mean ± SEM. Differences are significant for *P < 0.05.