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. 2015 Mar 18;10(3):e0119496. doi: 10.1371/journal.pone.0119496

Fig 1. Formation of an SPL/PP1 complex in platelets.

Fig 1

Lysates were prepared from platelets stimulated with the PAR1 agonist peptide, SFLLRN, after which PP1 precipitated with isoform-selective antibodies or nonimmune immunoglobulin (Ig) and probed with anti-spinophilin before re-probing with a pan-PP1 (E-9) antibody. The graphs for PP1α and γ summarize results from two experiments expressed as % of the result obtained at 3 min. No binding of PP1β was detectable at either time point after the addition of SFLLRN.