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. 2015 Mar 18;10(3):e0119496. doi: 10.1371/journal.pone.0119496

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© 2015 Ma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) Top: Lysates were prepared from CHO cells transfected with a full length, Myc-tagged spinophilin (Myc-SPL). Proteins were precipitated with an anti-Myc antibody or nonimmune immunoglobulin (Ig) and then probed for PP1 and Myc-SPL. Bottom: Lysates were prepared from mock-transfected control CHO cells or cells transfected with a full length, Myc-tagged spinophilin (Myc-SPL). Proteins were precipitated with anti-Myc and then probed for PP1 and Myc-SPL. (B) Lysates were prepared from CHO cells transfected with full length Myc-SPL and either PP1α, β or γ. Proteins were precipitated with PP1 isoform-specific antibodies and then probed for Myc-SPL or Flag-PP1 (mean ± SEM, N = 3). Note that all the samples were run on the same gel and, as indicated by the vertical gaps, two marker lanes were excised.