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. 2015 Mar 18;10(3):e0119496. doi: 10.1371/journal.pone.0119496

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© 2015 Ma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Human platelets were incubated for 3 min with 50 μM SFLLRN, a TxA₂ mimetic (U46619 10μM), ADP (10μM) or collagen (10μg/ml) in the presence of 100 μΜ aspirin (ASA), after which the samples were lysed and placed on ice. Lysates were precipitated with anti-pan-PP1 (antibody E-9) and probed for spinophilin (antibody A-20) before being re-probed for PP1 (antibody FL-18). The results shown are the mean ± SEM, N = 4.