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. 2015 Mar 18;10(3):e0119496. doi: 10.1371/journal.pone.0119496

Fig 6. Binding to spinophilin inhibits PP1.

Fig 6

(A) CHO cells were transfected with Myc-SPL and Flag-PP1α as indicated. Proteins were precipitated with anti-Myc and then probed with anti-PP1 (left) or assayed for SPL-associated phosphatase activity with a small molecule substrate (right) (mean ± SEM, N = 3). (B) CHO cells were transfected with PP1α and either Myc-tagged wild type or Myc-tagged F451A spinophilin as indicated. After immunoprecipitation with anti-Myc, precipitated proteins were probed with anti-PP1 and anti-Myc (mean ± SEM, N = 3). (C) Top and right. CHO cells were transfected with either wild type or F451A spinophilin and then immunoblotted for MLC and pMLC as indicated (mean ± SEM, N = 3). Bottom. Cell lysates were immunoblotted with anti-Myc, demonstrating equal expression of wild type and F451A spinophilin.