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. 2015 Mar 18;10(3):e0119879. doi: 10.1371/journal.pone.0119879

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© 2015 Schwalbe, Schreiber

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (a) For each NL-952.1, NL-952.2 and NL-952.3 virus all eight mutants (RRR-to-KKK) were tested for neutralization by SDF-1α. GHOST-X4 cells were infected with an amount of virus representing about 100 ffu. SDF-1α was added to a final concentration of 125, 250, 500 and 1000 ng/ml. (b) Neutralization by RANTES was carried out for X4R5-dualtropic NL-952.1 mutants with the exception of the X4-monotropic mutant RRR, since the RRR mutant does not show any viral growth in GHOST-R5 cells (infection = 0% at RANTES 0 ng/ml). Also all NL-952.2 and NL-952.3 mutants did not replicate in GHOST-R5 cells and therefore could not be tested for neutralization by RANTES.