Effects of pH and temperature on the tertiary structure of mALAS2. Near-UV CD spectra collected at different acidic and alkaline pH values at (A) 20 °C, and (B) 37 °C. (C) Spectra at 20 °C (solid) and 37 °C (dashed) at pH 7.5. (D) Spectra at 20 °C (solid) and 37 °C (dashed) at pH 9.5. The colors of the spectra are: pH 1 (pink), pH 2 (purple), pH 3 (gray), pH 7.5 (black), pH 8.5 (red), pH 9.5 (blue), and pH 10.5 (cyan).