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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Pflugers Arch. 2014 Sep 18;467(8):1723–1732. doi: 10.1007/s00424-014-1610-3

Fig. 1. Immunopurification of hTRPM4 from a plasma membrane-enriched protein fraction from tetracycline-induced (+) and non-induced (−) TREx-TRPM4 cells.

Fig. 1

a. SDS-PAGE separation of eluates from immunoprecipitation of TRPM4 with anti-FLAG antibody. Immunoblot of Input (I) and immunoprecipitation products (IP) of TRPM4 from TREx-TRPM4 cells after SDS-PAGE separation. b. Coomassie brilliant blue-stained SDS-PAGE of a large-scale immunopurification showing the yield of the FLAG-TRPM4 channels obtained with the anti-FLAG immunopurification from tetracycline-induced TREx-TRPM4 cells. The left lane corresponds to the molecular weight standards in kDa.