Skip to main content
. 2015 Mar 18;10(3):e0118434. doi: 10.1371/journal.pone.0118434

Fig 7. Macrophages are required for VLP-mediated post-exposure protection and IgG antibody production.

Fig 7

(A) CD11b-DTR mice were depleted of macrophages by injection of diphtheria toxin, then infected with EBOV and treated with VLPs 24 hours post-infection, or left untreated. As a control, wild-type mice were treated with diphtheria toxin and treated with VLP after infection. n = 4 for CD11bDTR+DT and CD11bDTR+DT+VLP groups, n = 10 for WT+DT+VLP group. (B) Mice were depleted of macrophages using clodronate encapsulated liposomes, then infected with EBOV and treated with VLPs 24 hours after infection. As a control, mice received PBS encapsulated liposomes before infection and VLP treatment. Additional controls include PBS- or clodronate-treated mice without VLP treatment. n = 19 for PBS; n = 20 for PBS+VLP; n = 15 for Clodronate; n = 16 for Clodronate+VLP; two separate experiments. (C) Clodronate-treated mice (or control PBS-treated mice) were infected with EBOV and treated with VLPs. On days 5, 6, or 7, sera were analyzed for anti-EBOV IgG and IgM responses.