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. 2015 Feb 12;7(3):299–314. doi: 10.15252/emmm.201404134

Figure 6.

Figure 6

Unlike wild-type NAV3, mutant cancer alleles are devoid of persistency induction and metastasis suppression
  • A MCF10A cells were plated on collagen, and 24 h later, they were treated with EGF (10 ng/ml). Shown are trajectories of cells transiently expressing the indicated forms of NAV3. Red tracks indicate relatively low persistence (< 0.6).
  • B Quantification of migration parameters (from A) is shown as mean ± SEM (60 cells and three experiments).
  • C HEK293 cells previously transfected with EGFP-NAV3 (WT), EGFP-NAV3-D1047N or EGFP-NAV3-A459P were treated with cycloheximide (CHX; 50 μg/ml) or with the solvent, DMSO, for the indicated time intervals, followed by cell lysis and immunoblotting using anti-EGFP and anti-α-tubulin antibodies. The signals obtained were quantified and normalized to time zero (untreated cells; right panel).
  • D, E The indicated mCherry-expressing MDA-MB-231 derivatives were injected intravenously (1.5 × 105 per mouse) using 5-week-old female SCID mice. Four weeks later, lungs were photographed and the numbers of lung metastases were determined.
Source data are available online for this figure.