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. 2015 Mar 18;10(3):e0119918. doi: 10.1371/journal.pone.0119918

Fig 4. LPS dependent increases in oxidative and nitrative stress are attenuated in endothelial NOS deficient mice.

Fig 4

The LPS mediated increase in nitric oxide (NOx) (A) and NOS-derived superoxide radical generation (B) was significantly lower in eNOS-/- mice compared to wild-type mice. Analyses of the levels of nitrative stress, estimated using both the peroxynitrite dependent oxidation of dihydrorhodamine (DHR) 123 to rhodamine 123 (C) and 3-nitrotyrosine (3-NT) levels using dot blot analysis (D), indicate that the LPS induced increase in peroxynitrite (C) and 3-NT (D) levels in the lungs of wild-type mice was absent in LPS treated eNOS-/- mice. Values are mean ± SEM, n = 6–10. *p<0.05 vs. Wild-type+Vehicle, †P<0.05 vs. Wild-type+LPS.