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. 2015 Mar 18;10(3):e0118424. doi: 10.1371/journal.pone.0118424

Table 1. Oligonucleotides used for the cloning of the reprogramming factors.

Gene Primer name (orientation), primer sequences (5ˈ → 3ˈ) Restriction enzymes
SOX2 G0028 (fwd): GCTAGGATCCACAGCGCCCGCATG BamHI/XhoI
G0030 (rev): CCGCTCGAGAATGCCTCCCCCGTCCAGTTCG
OCT4 G0022 (fwd): GATCGGATCCTTGGGGCGCCTTCCTTC BamHI/XbaI
G0023 (rev): CTGATCTAGACTCCTCTCCCTGTCCCCC
KLF4 G0020 (fwd): GTACGGATCCTGCGCAGCCACCTGGC BamHI/XbaI
G0021 (rev): GTACTCTAGACAGTGTGGGTCATATCCACTG
c-MYC G0077 (fwd): ATAAGAATGCGGCCGCAATGCCCCTCAACGTCAGCTTC NotI/SalI
G0078 (rev): ATGGCCGACGTCGACTTATGCACAAGAGTTCCGCAGC
LIN28 G0024 (fwd): GATCGGATCCGGCCACGGGCTCAGCCG BamHI/XhoI
G0025 (rev): GACTCTCGAGATAGCCAAAGAATAGCCCC
NANOG G0018 (fwd): GATCAAGCTTCCTTTTCCCCAATAATAACATG HindIII/XhoI
G0019 (rev): CTGACTCGAGTAGTGTCAGTTTCATTCATC