Endogenous factors |
SOX2
|
G0086 (fwd): TCTTCCTCGCACTCCAGGGC |
228 |
G0030 (rev): CCGCTCGAGAATGCCTCCCCCGTCCAGTTCG |
OCT4
|
G0022 (fwd): GATCGGATCCTTGGGGCGCCTTCCTTC |
510 |
G0035 (rev): CAGGGTGATCCTCTTCTGCTTC |
KLF4
|
G0091 (fwd): GGAAGACGATCTTGGCCCCG |
323 |
G0021 (rev): GTACTCTAGACAGTGTGGGTCATATCCACTG |
c-MYC
|
G0079 (fwd): ATAAGAATGCGGCCGCACTGGATTTTTTTCGGGCAGTGG |
456 |
G00142 (rev): CCTGGATGATGATGTTTTTGATG |
LIN28
|
G0305 (fwd): GACGAGCTGTACAAGGGGAGTGAGAGGCGGCCAAAGGGG |
334 |
G0025 (rev): GACTCTCGAGATAGCCAAAGAATAGCCCC |
NANOG
|
G0018 (fwd): GATCAAGCTTCCTTTTCCCCAATAATAACATG |
754 |
G0075 (rev): TTATAGAAGGGACTGCTCCAGG |
Reprogramming cassette |
SO (SOX2-OCT4)
|
G0086 (fwd): TCTTCCTCGCACTCCAGGGC |
735 |
G0035 (rev): CAGGGTGATCCTCTTCTGCTTC |
IC (IRES-Cerulean)
|
G0095 (fwd): AGCGACCCTTTGCAGGCAGC |
1073 |
G0039 (rev): GAAGATCTACTTGTACAGCTCGTCCATG |
PCAG-SOX2
|
G0244 (fwd): GGGGACGGCTGCCTTCGG |
190 |
G0378 (rev): GCTCGGTACCAAGCTTAAG |
PCAG-SOX2
|
G0244 (fwd): GGGGACGGCTGCCTTCGG |
361 |
G0395 (rev): CGGTCGGGGCTGTTCTTCTG |
NANOG-IRES
|
G0069 (fwd): GGTTCCAGAACCAGAGAATGAAATC |
576 |
G0098 (rev): CACCGGCCTTATTCCAAGCG |
Cerulean
|
G0618 (fwd): ACGTAAACGGCCACAAGTTCAGC |
211 |
G0619 (rev): CCTTCGGGCATGGCGGACTTG |
Control fragments |
mmSox2
|
G0656 (fwd): GGACATGATCAGCATGTACCTCC |
221 |
G0657 (rev): TCTCCTCTTTTTGCACCCCTCC |
β-ACTIN
|
G0336 (fwd): GACGACATGGAGAAGATCTGG |
562 |
G0337 (rev): GGAAAGAAGGCTGGAAGAGTG |
Real-time qPCR |
18S rRNA
|
G0871 (fwd): ATTAAGGGTGTGGGCCGAAG |
81 |
G0872 (rev): GAGTTCTCCTGCCCTCTTGG |
OCT4_endo (isoform A and B)
|
G0963 (fwd): GCCAGGGCTTTTAGGATTAAGTT |
68 |
G0964 (rev): TGCCCTCACCCTTTGTGTTC |
OCT4A_endo+exo
|
G0877 (fwd): CCCCTGGTGCCGTGAAG |
82 |
G0878 (rev): TTCTGCAGAGCTTTGATGTCTTG |
NANOG
|
G0965 (fwd): ATGCCACCTGAAGATGTGTGAA |
69 |
G0966 (rev): TCAGCCAGTGCTCAGAGTGAA |
Transposon
|
G0961 (fwd): AGCCAACATACTTTCGGGAGGA |
135 |
G0962 (rev): TACTCATTGGGCCAGGATTCTC |
SOX2
|
G0969 (fwd): TTGTTCAAAAAAGTATCAGGAGTTGTC |
97 |
G0970 (rev): CTCTCCGTCCCCGTCTTAAAG |
LIN28
|
G0682 (fwd): GCACAGGGAAAGCCAACATAC |
126 |
G0683 (rev): CGAAACTTCCTGATAGCCAAAGA |