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. 2015 Mar 18;10(3):e0120918. doi: 10.1371/journal.pone.0120918

Fig 4. Vγ9Vδ2 T cells are more sensitive to apoptosis and have a reduced ability to produce IFN-γ in response to nonobese APCs.

Fig 4

PBMC from non-obese (NOD) or obese (OD) donors were co-cultured with a monocytic cell line U937 to determine if the dysfunction was T cell intrinsic. (A, B) Representative plots of IFN-γ production by Vδ2+ CD3+ gated cells. PBMC were either cultured alone (uninfected), with U937 cells (U937 ctrl) or with influenza infected U937 cells (U937 infected). (C) Percentage of Vδ2+ T cells producing IFN-γ in NOD and OD in response to infected U937 cells (corrected for controls, n = 8). Horizontal lines represent the mean. (D) Change in MFI of CD69 expression on Vδ2+ CD3+ gated cells following 8 hour incubation with uninfected or infected U937 cells. Data represents five separate donor pairs and their standard deviation. P-values calculated from Pearson’s unpaired Student’s t-test. (E) Data represents the percentage of Vδ2+ T cells staining positive for Annexin-V but not PI (dark bars) or positive for both Annexin-V and PI (white bars) by flow cytometry (n = 5) with standard deviation.