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Lanes 1–4, lanes 5–8, lanes 9–12, and lanes 13–16 represent experiments performed with canonical H3, CENP-A, H3 mutant (with R49K and R53K), and CENP-A mutant (with K49R and K53R), respectively. The reaction periods are 0 min (lanes 1, 5, 9, and 13), 2.5 min (lanes 2, 6, 10, and 14), 5 min (lanes 3, 7, 11, and 15), and 10 min (lanes 4, 8, 12, and 16).
