Figure 8.

(A) Representative confocal images of proximity ligation assay (PLA). Nuclear proteins were extracted from THP-1 cells treated with LPS in the presence or absence of DIQ (300 μmol/L), a PARP1 inhibitor. THP-1 cells were treated with LPS (10 μg/mL) for 6 h. PLA amplification corresponds with the interaction of PARP1 with SIRT1 and is visualized as red-pink spots localized mainly in the nucleus. (B) Coimmunoprecipitation analysis from THP-1 cell lysates. Panel 1: Samples were immunoprecipitated with anti-SIRT1 and immunoblotted with anti-poly(ADP-ribose). The blot was then stripped and reprobed for SIRT1 (panel 2).