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. 2015 Jan 20;20(1):516–526. doi: 10.2119/molmed.2014.00057

Figure 1.

Figure 1

Schematic overview of the experimental protocols. (A) Experimental setup for histological analysis. Rats were euthanized after 25 min (25′) of ischemia and 5, 15, or 30 min (5′, 10′, or 30′) of reperfusion (groups I/R5, I/R15, I/R30; the group I had ischemia alone; the sham group had no I/R. The number behind the reperfusion block indicates the duration (in minutes) of the reperfusion phase. Helium postconditioning (He) encompassed the entire reperfusion phase (groups He5, He15, He30). (B) Experimental setup for gene analysis by qRT-PCR. Rats were euthanized after 25 min of ischemia or after 15 min of reperfusion.