Table 1.

Streptomyces strains and plasmids used in this study.

Strain	Genotype	Pgl status	References
M145	Prototrophic	+	Kieser et al. (2000)
J1934	Insertional mutation of PglZ	−	Bedford et al. (1995)
SLMW	ΔpglW M145 isogenic	−	Khodakaramian et al. (2006)
SPHX	ΔpglX M145 isogenic	−	This work
SLMY	ΔpglY M145 isogenic	−	Khodakaramian et al. (2006)
SPHZ	ΔpglZ M145 isogenic G-tract mutant	−	This work
SPHWZ	ΔpglWZ M145 isogenic G-tract mutant	−	This work
SPHXZ	ΔpglXZ M145 isogenic G-tract mutant	−	This work
SPHYZ	ΔpglYZ M145 isogenic G-tract mutant	−	This work
Plasmid	Description		Reference
pIJ6902	Integrating vector with thiostrepton-inducible promoter		Huang et al. (2005)
pT7-7	E. coli expression plasmid		Tabor and Richardson (1985)
pPS8002	pglW–His6 in pIJ6902		Sumby and Smith (2002)
pPS8003	pglX–His6 in pIJ6902		Sumby and Smith (2002)
pPS8008	His6–pglY in pIJ6902		Sumby and Smith (2002)
pPH1001	pglZ–His6 in pIJ6902		This work
pPH1002	pglXY381A–His6 in pIJ6902		This work
pPH1003	His6–pglYK81A,S82A in pIJ6902		This work
pPH1005	pglZD535A–His6 in pT7-7		This work
pPH1006	pglZD694A–His6 in pT7-7		This work
pPH1007	pglZD535A–His6 in pIJ6902		This work
pPH1008	pglZD694A–His6 in pIJ6902		This work
pPH1010	His6–pglYK81A,S82A in pT7-7		This work
pPH1012	pglWK677A–His6 in pIJ6902		This work
pPS5032	pglX–His6 in pT7-7		This work
pPS5045	pglZ–His6 in pT7-7		This work
pPS5012	pglW–His6 in pT7-7		This work
pPS5025	pPS5012 with optimised codons in pglW		This work
pPS5072	His6–pglY in pT7-7		This work