Table III.

The Effect of the Numbers of Replicates on the Cost of Absolute Quantification Proteomics Analysis of Human Tissue Samples

Number and type of replicate	Label-free	AQUA	QconCAT
3 biological replicates for the analysis of 1 protein	$788	$1,106	$4,941
Technical replicates for each biological replicatea	$709	$497	$1,755
Biological × technical replicates for the analysis of 1 protein	$2,126	$1,490	$5,325
Biological × technical replicates for the analysis of 50 proteins	$2,126	$27,841	$6,025
Biological × technical replicates for the analysis of 500 proteins	$2,126	$271,282	$31,908

^aThe values are based on two technical replicates for AQUA and QconCAT, and three technical replicates for label-free methods