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. 2014 Aug 27;5:4778. doi: 10.1038/ncomms5778

Figure 4. GFP–TipAct allows growing microtubules to transport, pull and bundle actin filaments, globally dictating F-actin organization.

Figure 4

(a) Time series of a growing microtubule end that transports a short actin filament (Supplementary Movie 8). Schematic of this effect (bottom). (b) Time series of a growing microtubule that aligns and then pulls on an actin filament (Supplementary Movie 9). The dashed yellow line in the actin pane shows the contour changes of the actin filament. Arrowheads in the merge pane show the microtubule plus-end. Schematic of this effect (bottom). (c) Time series of a growing microtubule that captures actin filaments and forms a bundle, which then guides the growth of another microtubule (Supplementary Movie 11). The dashed yellow line in the actin pane shows the formation of the bundle. White arrowheads and arrows in the merge pane show the growing plus-ends of these two microtubules. Schematic of this effect (bottom). Steady-state F-actin organization in the vicinity of a radial array of dynamic microtubules, with (e), and without (d) GFP–TipAct. Composition in a and b: 16 μM tubulin, 100 nM EB3 and 50 nM GFP–TipAct for n=8 and 9 experiments, respectively; in c: 20 μM tubulin, 100 nM EB3 and 25 nM GFP–TipAct for n=10 experiments; in d and e: 20 μM tubulin, 100 nM EB3, with (e) and without (d) 200 nM GFP–TipAct for n=20 and 5 experiments, respectively. Scale bars, 5 μm (a–c); 10 μm (d,e); time, min:s. MT, microtubule.