TABLE 2.
Sequences of the primers and probes for the A4L and A36R assaysa
| Primer or probe | Sequence for: |
|
|---|---|---|
| A4L | A36R | |
| Forward | 5′ ACATTCATCCTATTCTGATGGCA 3′ | 5′ TTCAGACTACCAGTTATTCTCGGA 3′ |
| Reverse | 5′ TGCTGGATTAGACTATATGTGTCTCC 3′ | 5′ AGCCAGGTAGTCAAGACATCAGA 3′ |
| Probe | 5′ FAM-TTTGGCATTAAACTTCCCGCGCTT-BHQ 3′ | 5′ FAM-CAAATTGCGCCACAGAATCATCAAC-BHQ 3′ |
a
Primer and probe sets were synthesized by the Biotechnology Core Facility (CDC, Atlanta, GA). The sequences are from BSH75 banu (GenBank accession number DQ437581). The amplicon lengths are 94 bp for A4L and 93 bp for A36R.