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Master mix reaction mixture for both the A4L and the A36R assays
| Component | Amt and concn |
|---|---|
| LightCycler FastStart DNA Master HybProbe master mix (Roche Applied Science) | 2.5 μl of 10× mix |
| Forward primer | 1 μl of 25 μM stock |
| Reverse primer | 1 μl of 25 μM stock |
| Probe | 1 μl of 25 μM stock |
| Magnesium chloride | 3 μl of 25 mM stock |
| Sample DNA | 5 μl |
| Total vol | 25 μl |
