TABLE 2.
Laboratory investigations performed on the two discrepant M. tuberculosis isolates
| Laboratory investigations | Data for M. tuberculosis isolatea: |
|
|---|---|---|
| Kw5741-13 | Kw9101-13 | |
| Clinical specimen | Pus | Sputum |
| Smear microscopy result | AFB negative | AFB negative |
| Xpert MTB/RIF assay result for specimen | Presence of RMP-resistant M. tuberculosis | Presence of RMP-resistant M. tuberculosis |
| MGIT 960 system culture result | Positive | Positive |
| RMP susceptibility (1 mg/liter) | Susceptible | Susceptible |
| Other resistance pattern | None | None |
| PCR for M. tuberculosis complex | Positive | Positive |
| GenoType MTBDRplus assay | RMP resistant (ΔWT5, no specific mutation) | RMP resistant (ΔWT3 & 4, no specific mutation) |
| Sequencing of rpoB gene | CTG521TTG (silent mutation [Leu521Leu]) | GAC to TAC (missense mutation [Asp516Tyr]) |
a
ΔWT, lack of hybridization with the wild-type probe.