TABLE 1.

Minimized cost of fluorescent PCR ribotyping reagents

Purchased item	Vendor	List price ($)	Units per 96-well plate	Shipping cost ($)	Cost per 96-well plate ($)
PCR reagent
Forward primer (standard desalting)	IDTDNAa	7.00	46.04	3.75	0.23
FAM-labeled reverse primer (standard desalting)	IDTDNA	73.20	132.29	3.75	0.58
96-well PCR plate	VWR International	315.10	100		3.15
Promega PCR master mix	Fisher Scientific	88.40	2.08		42.43
Standard reagent and supplies
Bar-coded 96-well plate	BioExpress	1,412.35	500	67.50	2.96
Hi-Di Formamide	Life Technologies	36.45	21.48	61.50	4.56
MapMarker X-Rhodamine size standard	BioVentures	335.00	80	29.38	4.55
Fragment analysis
Shipping to sequencing core facility					16.17
CE fragment analysis on ABI3730XL					52.50
Total cost per 96-well plate					127.15
Total cost per well					1.32

^aIDTDNA, Integrated DNA Technologies.