Table 2.
Molecularly identified EM fungi on Salix alba, Salix caprea, and Betula pendula fine roots during autumn 2012 and spring 2013
| Tree species and season | T bp | Closest BLAST match (accession numbers—NCBI* and/or UNITE**) | % similarity | Classified as | EM density |
|---|---|---|---|---|---|
| Autumn 2012 | |||||
| S. alba | 637 | Tomentella isolate [EU668202 and UDB002428]* (Bidartondo and Read 2008); Tomentella stuposa [UDB011637]** | 617/630 (97 %) and 587/595 (98 %) 623/639 (97 %) | Tomentella sp. Sa-A [KP745605] | 24.63 % |
| S. caprea | 699 | Hebeloma collariatum [JQ724066]* | 693/696 (99 %) | H. collariatum Sc-A [KP745607] | 3.88 % |
| H. collariatum [UDB015489]** | 668/674 (99 %) | ||||
| 636 | Geopora sp. [JQ724044]* (Hrynkiewicz et al. 2012b) | 565/636 (89 %) | Geopora sp. Sc-A [KP745606] | 11.82 % | |
| Geopora arenicola [UDB017620]** | 368/406 (90 %) | ||||
| B. pendula | 563 | Uncultured fungus genomic [FN397282]* (Napoli et al. 2010) | 563/566 (99 %) | Helotiales sp. Bp- [KP745604] | 27.18 % |
| Ectomycorrhizal fungus [JX043062]* (Karst et al. 2013) | 542/552 (98 %) | ||||
| Helotiales sp. [JN859267]* (Knapp et al. 2012) | 528/541 (98 %) | ||||
| Spring 2013 | |||||
| S. alba | 874 | Thelephoraceae [EF218829]* (Twieg et al. 2007) | 831/874 (95 %) | Tomentella sp. Sa-S [KP745608] | 10.82 % |
| Thelephoraceae [AJ893343]* (Tedersoo et al. 2006b) | 800/831 (96 %) | ||||
| Tomentella sp. [UDB018687]** | 832/866 (96 %) | ||||
| T. ellisii [UDB016490]** | 818/855 (95 %) | ||||
| S. caprea | 623 | Tomentella ellisii [DQ068971]* (Menkis et al. 2005) | 598/614 (97 %) | Tomentella sp. Sc-S [KP745609] | 7.54 % |
| Tomentella sp. [UDB018687]** | 583/614 (94 %) | ||||
| T. ellisii [UDB016490]** | 569/603 (94 %) | ||||
| B. pendula | – | Colour, brownish; rhizomorphs, not observed; mantle, plectemchymatic B; cystidia, lacking; surface, smooth; emanating hyphae, scarce | – | Thelephoraceae B.p_1S | 6.95 % |
| – | Colour, brown; rhizomorphs, not observed; mantle, plectemchymatic B; cystidia, lacking; surface, smooth; and emanating hyphae, lacking | – | Pyronemataceae B.p_2S | 5.20 % | |
| – | Colour, gold-brown; rhizomorph,: not observed;, mantle, plectemchymatic A; cystidia, lacking; surface: smooth; emanating hyphae: abundant with clamps | – | Thelephoraceae B.p_3S | 1.97 % | |
Abbreviations: B.p Betula pendula, S.c Salix caprea, S.a Salix alba