Table 2.
Antigen | Clone | Dilution | Pretreatment | Primary antibody incubation time | Source |
---|---|---|---|---|---|
AID | — | 1 : 50 (rabbit polyclonal) | MW | Overnight (about 15 h, 4°C) | Serotec |
LMP-1 | CS. 1–4 | 1 : 100 (mouse monoclonal) | — | Overnight (about 15 h, 4°C) | Dako |
Egr-1 | — | 1 : 100 (rabbit polyclonal) | — | Nonovernight (1 h, RT) | Rockland |
AID: activation-induced cytidine deaminase; LMP-1: latent membrane protein-1; Egr-1: early growth response-1; MW: microwave oven (for 1 min at high voltage and then for 10 min at low voltage); —: none; RT: room temperature.