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. 2015 Mar 19;5:9292. doi: 10.1038/srep09292

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(a) Gel retardation assay showing 250 ng naked pCI-Luc, LD (0.75:1), LD (4:1), PD, and LPD complexes incubated for 1 hour at room temperature in the presence or absence of 20 U/mL heparan sulphate. (b) PicoGreen fluorescence quenching was used to assess the stability of the different complexes to heparan sulphate challenge as well as assess the quenching capabilities of the different complexes in the absence of heparan sulphate. The level of PicoGreen fluorescence was measured in relative fluorescence units (RFU) with naked PicoGreen labelled pCI-Luc plasmid set as 100% RFU. Values are mean ± SEM; n = 6.