Table 4. Functional survival* of oocytes vitrified in 0.72 or 1.0 molal sucrose + isotonic PB1 + Ficoll as a function of the fraction of cell water at the time of vitrification.
(a) | (b) | (c) | (d) | (e) | (f) | (g) | ||
---|---|---|---|---|---|---|---|---|
No. | Solution | Total external molality | Vwl (~Ww) after 2 min | Rel mass of cell solids | Mass of cell water @ 2′/mass of solids | Mass of cell water/Total mass @ 2 min | % Survival with laser warming (1 × 107°C/min) | Survival w/o laser warming (1.2 × 105°C/min) |
11 | 0×Std-1 | 0.88 | 0.291 | 0.233 | 1.25 | 0.555 | 78 ± 6.5 | 0 |
12 | 0×Std-1a | 0.87 | 0.294 | 0.233 | 1.26 | 0.558 | 84 ± 3.5 | 0 |
13 | 0×Std -2 | 1.156 | 0.260 | 0.233 | 1.12 | 0.527 | 96 ± 3.6 | 0 |
*Survivals are based on the percentages of morphologically normal oocytes developing to 2-cell embryos after in vitro fertilization. Morphological survivals were 77% and 83% in 0.72 and 1.0 molal sucrose, respectively.
Column (c): The solutions contained 0.72 or 1.0 molal sucrose plus 0.15 molal PB1 salts and 0.0013 or 0.0062 molal Ficoll.
See footnotes in Table 3 for the procedures used to calculate Columns (d), (f), and (g).