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. 2015 Mar 19;5:9271. doi: 10.1038/srep09271

Table 4. Functional survival* of oocytes vitrified in 0.72 or 1.0 molal sucrose + isotonic PB1 + Ficoll as a function of the fraction of cell water at the time of vitrification.

(a) (b) (c) (d) (e) (f) (g)    
No. Solution Total external molality Vwl (~Ww) after 2 min Rel mass of cell solids Mass of cell water @ 2′/mass of solids Mass of cell water/Total mass @ 2 min % Survival with laser warming (1 × 107°C/min) Survival w/o laser warming (1.2 × 105°C/min)
11 0×Std-1 0.88 0.291 0.233 1.25 0.555 78 ± 6.5 0
12 0×Std-1a 0.87 0.294 0.233 1.26 0.558 84 ± 3.5 0
13 0×Std -2 1.156 0.260 0.233 1.12 0.527 96 ± 3.6 0

*Survivals are based on the percentages of morphologically normal oocytes developing to 2-cell embryos after in vitro fertilization. Morphological survivals were 77% and 83% in 0.72 and 1.0 molal sucrose, respectively.

Column (c): The solutions contained 0.72 or 1.0 molal sucrose plus 0.15 molal PB1 salts and 0.0013 or 0.0062 molal Ficoll.

See footnotes in Table 3 for the procedures used to calculate Columns (d), (f), and (g).