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. 2015 Mar 19;5:9330. doi: 10.1038/srep09330

Figure 2. Increased CXCL13-dependent chemotaxis of MCF-7 breast cancer cells upon p53 knock-down.

Figure 2

(A) MCF-7 cells did not migrate under the control spots containing IL7 (A, top left) and showed a low level of chemotactic activity towards recombinant CXCL13 (A, bottom left). MCF-7-2Si cells still showed no migration activity under control spots with IL7 (A, top right) but migrated under spots with CXCL13 more readily (A, bottom right). Yellow dashed lines show the borders of the spots, yellow arrows indicate the areas of cell migration. Similar results were obtained with purified recombinant CXCL13, however, the migration of MCF-7-2Si cells was even more aggressive (data not shown). (B) Average migration rates of MCF-7 and MCF-7-2Si cells under agarose spots were estimated by counting cells under 10 spots of similar radius. The experiment was repeated 6 times. (C) Chemotactic activity of MCF-7 and MCF-7-2Si cells in quantitative cell migration assay with ThinCert cell culture inserts. The data shown is the result of three replicate experiments. *P < 0,01.