Figure 2. Validation of a representative set of 14 SSR markers (physically mapped on eight chickpea chromosomes) showing in silico fragment length polymorphism between parental genotypes (ICC 4958 and ICC 12968) of a F₄ mapping population (ICC 4958 × ICC 12968) using the gel-based assay (a) and fluorescent-dye labeled automated fragment analyzer (b). (c) Segregation pattern of one selected SSR marker in a representative set of mapping individuals. (d) Amplification and polymorphism profiles of one SSR marker in a selected set of desi and kabuli genotypes.

The fragment sizes (bp) of the amplified polymorphic alleles are indicated. The identities of SSR markers with their detailed information are provided in the Supplementary Table S1. M: 50 bp DNA ladder size standard.