Figure 3. Assessment by FACS analysis of FPR-1 and FPR-2 receptor internalization in PMNs produced by cromones.

Human PMN were incubated with either nedocromil (10nM) or dexamethasone (2nM) for 5min at 37°C; after immediate transfer to 4°C, FPR1 and FPR2 cell surface expressions were determined with specific antibodies and flow cytometry analysis. Data are presented as mean ± SEM of 3 independent experiments, ^*p<0.05 vs control group (see the Supplemental Materials file for details).